Ctb toxin
WebFeb 11, 2024 · Cholera toxin produced by Vibrio cholerae is usually an infectious agent of the gut, but the toxin also has the potential to be developed for more versatile tracing and targeting of neurons. It is … WebDescription. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1, making it powerful tool for retrograde labeling of neurons; This tracer has been used in variety of applications, including tracing of rat forebrain afferents, projections of parabrachial region, and neurons of urinary bladder wall
Ctb toxin
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WebJul 23, 2024 · CTB FITC and CTB biotin Conjugates of cholera toxin B subunit have been employed as histochemical markers in both axoplasmic transport and neuronal … Because the B subunit appears to be relatively non-toxic, researchers have found a number of applications for it in cell and molecular biology. It is routinely used as a neuronal tracer. Treatment of cultured rodent neural stem cells with cholera toxin induces changes in the localization of the transcription factor Hes3 and increases their numbers. GM1 gangliosides are found in lipid rafts on the cell surface. B subunit complexes labelled with fl…
WebMar 1, 2024 · CTB is wildly used as a nerve probe or to deliver therapeutic drugs to nerve which is rich in GM1 receptor. Compared to existing local injection, noninvasive administration of CT-based carrier is friendly to patients. ... Red fluorescent AuNDs with conjugation of cholera toxin subunit B (CTB) for extended-distance retro-nerve … WebDec 7, 2015 · The cholera toxin B subunit (CTB) binds to cellular surfaces via its receptor monosialoanglioside (GM1) . As a conventional bacterial toxin for neuronal tracing, CTB enters the axons or dendrites by active …
WebJul 24, 2015 · The dual protein nature of cholera toxin was initially recognized by Lönnroth and Holmgren in 1973 , and structural studies of the closely related heat-labile … WebThe most common shorthand of "cholera toxin beta" is CTB. You can also look at abbreviations and acronyms with word CTB in term. Page Link; Citation Styles; Suggest …
WebBinding of cholera toxin B subunit (CTB) to ganglioside GM1 is a marker to identify lipid rafts. Primary human T cells were isolated from healthy donors and were stimulated with superantigen staphylococcus enterotoxin B (SEB) and stained with cholera toxin B-fluorescein isothiocyanate (CTB-FITC). An optimized staining procedure is required to ...
WebFeb 15, 2024 · Host cell surface molecules are recognized by the B subunit, facilitating cell entry by the A subunit, which activates adenylate cyclase, thereby leading to massive ion and fluid secretion. In the early 1970s, the ganglioside GM1 was identified as a high-affinity binding partner for cholera toxin subunit B (CTB) [2,3]. philosophy\\u0027s 0tWebCholera Toxin Subunit B Specifications: • Label (Ex/Em): Alexa Fluor™ 488 (495/519 nm) • At neutral pH, the 11.4 kDa B subunit exists as a 57 kDa pentamer. • Lyophilized product can be dissolved in buffer (e.g., PBS) for use. Cholera Toxin Subunit B for Studying … Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1, … Molecular Probes™ cholera toxin conjugates are made from a … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes philosophy\u0027s 09WebCholera Toxin B subunit (CTB); FITC conjugate, lyophilized powder; This compound is a featured product for Cyclic Nucleotide research; The cholera toxin B subunit is used for … t shirt printing west islandWebDescription. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside G M1, making it powerful tool for retrograde labeling of neurons. This tracer has been used … philosophy\\u0027s 0yWebThe detailed contribution of the monosaccharides in GM1os to the CTB binding has been evaluated and the terminal Gal and NeuAc were found to be the primary donors to the intrinsic binding energy (54% and 44%, respectively).The contribution of the terminal Gal on the CTB–GM1os affinity arises both from the stacking with a tryptophan residue (Trp 88) … philosophy\\u0027s 14WebDec 7, 2015 · Cholera toxin B subunit (CTB) has been extensively used in the past for monosynaptic mapping. For decades, it was thought to lack the ability of transneuronal … philosophy\u0027s 13WebMar 15, 2024 · Cholera toxin B subunit (CTB, approx. 58 kDa), is a nontoxic component of the cholera toxin (CTX) released by Vibrio cholerae, a Gram-negative bacterium causing profuse secretory diarrhea 1. philosophy\u0027s 14