WebOct 22, 2024 · BBTools是用于DNA和RNA测序reads的比对工具,大多数小工具基于shell、java组成,可以处理像Illumina,454,Sanger,Ion Torrent,Pac Bio和Nanopore等数据,支持fastq,fasta,sam,scarf,fasta + qual,压缩或raw文件格式,几乎涵盖了我们常用的诸多操作,BBTools也是开源的,可以无限制免费使用,总体来说常常处理测序数据的小 … WebJul 14, 2024 · It was masked in 3 ways, using a program I developed called BBMask: 1) Areas containing multiple repeat short kmers. 2) Windows of low entropy, calculated using pentamer frequencies. 3) Via mapping from sam files: …
Yes .. BBMap can do that! - Part I : bbmap (aligner), bbduk …
WebMay 17, 2024 · i have a big difference between results using bbduk.sh and trimmomatic trimming single-end reads, i have used the commands below, trimmomatic kept 99.78% survival reads whereas bbduk 91.76%. I don't know which to consider good or not. WebApr 28, 2015 · I currently use BBmap to trim Illumina addapter using the command: bbduk.sh -Xmx1g in1=f1.fq in2=f2.fq out1=clean1.fq out2=clean2.fq ktrim=r ref=truseq_rna.fa.gz k=28 mink=12 hdist=1. The f1.fq and f2.fq are from Illumina 1.9. When I check the output clean1.fq and clean2.fq files using FastQC. There is still evidence of … gzip python 解凍
Yes .. BBMap can do that! - SEQanswers
WebMar 24, 2024 · Can also trim (remove) the matching parts of the reads rather than binning the reads. Please read bbmap/docs/guides/BBDukGuide.txt for more information. Usage: bbduk.sh in= out= ref= Input may be stdin or a fasta or fastq file, compressed or uncompressed. WebOct 26, 2015 · BBDuk can process fasta, fastq, scarf, qual, and sam files, raw, gzipped, or bzipped. It can also handle references that are very large (even the human genome) in a … WebThis package includes BBMap, a short read aligner, as well as various other bioinformatic tools. It is written in pure Java, can run on any platform, and has no dependencies other than Java being installed (compiled for Java 6 and higher). All tools are efficient and multithreaded. BBMap: Short read aligner for DNA and RNA-seq data. gzip rsyncable